Restriction enzymes and their isoschizomers.

Since the last compilation of restriction enzymes, (1) 442 new entries have been added including 13 new specificities. A complete list of these new enzymes can be found in Table I. With the growing size of the restriction enzyme database and the recognition that the most widespread use of the information is as a database for computer programs predicting restriction enzyme cleavage patterns, Table n contains a listing of all prototype restriction enzymes (Types I, II and HI) together with their commercially available isoschizomers. It should be noted that an alternative compilation of these enzymes has recently been produced (3). During the previous year the names of several restriction enzymes have been changed by the original authors.